SANS studies of phospholipid:sterol vesicles and the membrane interactions of the anti-fungal drug, amphotericin

DOI

The increasing incidence of pathogenic fungi resistant to treatment with amphotericin B (AmB) has created a demand for novel anti-fungal agents that can circumvent the pathogens¿ resistance, and the successful development of such compounds will clearly require an understanding of the drug¿s mechanism of action at the molecular level. It has long been held that AmB exerts its anti-fungal action through the generation of self-assembled ion channels formed in the ergosterol-containing fungal cell membranes, but not in the cholesterol-containing human cell membranes. There is no direct structural evidence to support this hypothesis, however, and recent research suggests it is seriously flawed. In the SANS studies proposed here, we seek to understand how differences in a sterol¿s chemistry influence its effects on biomembrane structure, and how these then affect AmB membrane interactions.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24032879
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24032879
Provenance
Creator Dr Dave Barlow; Ms Fabrizia Foglia; Dr Hisham Al-Obaidi; Professor Jayne Lawrence
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2008-12-01T09:09:39Z
Temporal Coverage End 2009-07-29T08:49:50Z