Neutron reflectivity studies of the interaction of amphotericin with model cell membranes


The increasing incidence of pathogenic fungi resistant to treatment with amphotericin (AmB) has created a demand for novel anti-fungal agents that can circumvent the pathogens¿ resistance, and the successful development of such compounds will clearly require an understanding of the drug¿s mechanism of action at the molecular level. It has long been held that AmB exerts its anti-fungal action through generation of self-assembled ion channels formed in ergosterol-containing fungal cell membranes, but not in cholesterol-containing human cell membranes. There is no direct structural evidence to support this hypothesis, however, and recent research suggests it is seriously flawed. In the reflectivity studies proposed here, we seek to understand how differences in a sterol¿s chemistry influence its effects on biomembrane structure, and how this then affects the membrane interactions of AmB.

Metadata Access
Creator Dr Rob Barker; Professor Jayne Lawrence; Dr Dave Barlow; Dr Aleksandra Dabkowska; Ms Fabrizia Foglia; Dr Arwel Hughes; Dr Devashi Adroja
Publisher ISIS Neutron and Muon Source
Publication Year 2013
Rights CC-BY Attribution 4.0 International;
OpenAccess true
Contact isisdata(at)
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2010-03-21T01:26:38Z
Temporal Coverage End 2010-08-01T08:00:58Z