Further SANS contrast measurements on important protein/DNA interactions from RCaH

DOI

This proposal is to perform further SANS contrast measurements on three protein/DNA interactions currently worked on at the Research Complex at Harwell. This is part of an ongoing productive relationship between the two facilities, coordinated by Dr. David Scott of the University of Nottingham who is currently on sabbatical leave for 2 days at week at the RCaH.The systems understudy all form part of crystallographic studies as part of an MRC programme grant to Prof. Simon Phillips, the RCaH Director, and all are important in the fundamental processes of DNA repair, recombination and replication. Preliminary data is available from SAXS and analytical ultracentrifugation measurements showing that the systems are stable in D2O and monodisperse. Preliminary SANS data through SANSexpresses has shown that suitable concentration ranges are attainable for further SANS measurements.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24090590
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24090590
Provenance
Creator Dr David Scott; Mr Matthew Green; Dr Stephen Carr
Publisher ISIS Neutron and Muon Source
Publication Year 2016
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2013-05-20T09:04:47Z
Temporal Coverage End 2013-05-23T07:33:56Z