Molecular Mechanistic Processes of Complexation between DNA and Cationic Copolymers


It is difficult for the naked external gene to become internalized due to its similar charge to the extracellular membrane surface. Various cationic copolymers and cationic lipids have been reported to show different effects, but the exact mechanistic processes remain unclear. Polymers may interact with DNA to form DNA polyplexes through hydrophobic, entropic and electrostatic interactions. The net charge, size and structure of the complexes formed may influence cell transfection efficiency, but it remains largely unknown about how to control the gene-polymer complexation to optimize cell transfection and cytotoxicity. This work aims to rationalize the molecular processes by investigating complexation between a model diblock cationic copolymer and DNA.

Metadata Access
Creator Professor Jian Lu; Ms Amy Freund; Ms Donghui Jia; Dr Mohammed Yaseen
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International;
OpenAccess true
Contact isisdata(at)
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-12-04T09:37:27Z
Temporal Coverage End 2009-12-07T08:10:18Z