The Interaction of wild-type and mutant Puroindoline B with Model Bacterial Membranes

DOI

Bacterial and fungal infections of cereal crops pose a considerable risk to global food security. Typically plant pathogens are controlled through the use of chemical agents including fungicides and pesticides, to which resistance is becoming widespread. Increasingly attention is now being turned to the role that naturally occurring antimicrobial proteins can play in defence against disease-causing agents. We are studying one such protein, called puroindoline, which is found in wheat. We wish to use neutron reflectivity to understand better how it penetrates the surface of microbial and fungal cells and what controls how it does this in terms of the structure of the protein and of different types of cell surfaces.

Identifier
DOI https://doi.org/10.5286/ISIS.E.82369434
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/82369434
Provenance
Creator Professor Anthony Watts; Ms Marleen Wilde; Dr Luke Clifton; Dr Peter Judge; Mr Daniel Yin; Professor Rebecca Green; Professor Richard Frazier
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-09-18T08:00:00Z
Temporal Coverage End 2016-09-21T08:00:00Z