The Formation of Ion Channels in Phospholipid Bilayers by Puroindoline-a

DOI

Puroindolines are basic, cysteine rich proteins of ~13KDa, which have attracted significant interest due to their role in determining the endosperm texture of Wheat and seed defense. The antibacterial activity of both Pin-a and -b has been shown to be related to their ability form ion channels across both fungi and bacterial membranes. Pin-a is highly organized in solution, which is unusual for lipid binding/membrane proteins and suggests at the pathogenic membrane interface the protein is able to reorganize itself to enable penetration across the bilayer. Here, we wish to use neutron reflection to examine the interaction of Pin-a with both lipid monolayers and floating bilayers. We will use examine the interaction of Pin-a with model bilayers and monolayers of DPPG, Gaining insights into the activity of this biotechnologically important protein family.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24069236
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24069236
Provenance
Creator Dr Rob Barker; Dr Arwel Hughes; Dr Cameron Neylon; Dr Luke Clifton; Professor Rebecca Green; Professor Richard Frazier
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-06-14T08:17:10Z
Temporal Coverage End 2009-06-17T03:43:49Z