Surfactants are frequently used in personal care products to improve the wetting and oil solubilizing qualities of the products, which also leads to the skin irritation by damaging the barrier properties of the stratum corneum and denaturing proteins in the epidermis and the dermis. Lipid is known as the main component of skin, thus, for surfactant-induced skin irritation, interaction of skin lipid with surfactants should be firstly considered. Here we want to study the interaction between different surfactants and phospholipid vesicles, which aims to make a comprehensive understanding of the role of hydrophobic chain fluidity in the lipid-surfactant interaction.