Neutron Reflectivity Study of Protein Adsorption onto Cell Membrane-inspired Anti-Fouling Phospholipid Polymer Brush Surface

DOI

We grafted 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer brush from the silicon wafer surface through Cu (0) mediated controlled radical polymerization (CuCRP) to mimic cell membrance. Excellent control over graft density and film thickness was acheived by reaction condition variation. An optimal thickness of PMPC for protein resistance was estabilished. The adsorption then was found to increase together with the PMPC thickness. This can be either attributed to an increased surface adsorption or to protein penetration within the structure.In order to progress our understanding of the system further, we need to determine the protein distribution across the PMPC layer as a function of both protein concentration and graft density. Because of the advantage offered by contrast variation and the Ångstrom resolution, neutron reflectivity is the ideal technique to study these systems.

Identifier
DOI https://doi.org/10.5286/ISIS.E.88602240
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/88602240
Provenance
Creator Dr Mario Campana; Dr Aleksandra Szczes; Professor Emil Chibowski; Miss Yingdi Yan
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2017-10-11T23:00:00Z
Temporal Coverage End 2017-10-17T07:22:52Z