Of concern to the bioprocessing industry is the manner in which polysorbates included in solution formulations of antibodies affect the kinetics and equilibrium state of antibody adsorption/desorption to hydrophilic (glass) and hydrophobic (plastics) surfaces. Work with MedImmune has investigated this scenario using total internal reflection fluorescence (TIRF), showing that the time taken to reach equilibrium and the final amount of antibody adsorbed is strongly dependent on the shear rate and bulk concentration of antibody and polysorbate. Solid state circular dichroism showed that antibody structure was lost upon adsorption to hydrophobic surfaces. We propose to obtain high resolution data for adsorbed antibody over a range of concentrations in order to interpret kinetic data for antibody adsorption with/without polysorbate acquired over 120 s timescales to fit with the TIRF data.