Temperature Effects and Kinetics of Thiolipid Adsorption to Gold Surfaces


We have a programme investigating the structure and function of protein scaffolds oriented on gold surfaces using sulfur chemistry for attachment. The aim is to direct the self-assembly of membrane protein arrays retaining the protein's three dimensional structure and native membrane orientation. Both of these aspects are crucial to the full functionality of a device. The geometry of the system is ideally suited to study by neutron reflection. The system is somewhat more complex than that typically formed by a peptide fragment or surfactant. In addition to the protein's complex shape there is also the insulating bilayer surrounding the protein that is crucial to functionality. Recent experiments at NIST have revealed that temperature is an important variable in the bilayer deposition. This proposal will investigate the impact of temperature on kinetics and the lipid layer structure.

DOI https://doi.org/10.5286/ISIS.E.24003086
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24003086
Creator Dr Majkrzak; Dr Stephen Holt; Professor Jeremy Lakey; Dr Anton Le Brun
Publisher ISIS Neutron and Muon Source
Publication Year 2011
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Resource Type Dataset
Discipline Chemistry; Natural Sciences
Temporal Coverage Begin 2008-03-03T09:19:57Z
Temporal Coverage End 2008-03-06T22:44:09Z