Further Studies on the Gating Mechanism of an Antimicrobial Peptide Using Contrast Matched Bilayers

DOI

Antibiotic peptides are generally small, simple, usually alpha-helical peptides, which nevertheless show quite complex biological behaviour. We have been studying the gating mechanism of a simple, model antimicrobial peptide using neutron reflection. Whilst we have seen changes in our reflectivity profiles consistent with gating in our previous measurements, we have not been able to unambiguously show that the published proposed mechanism is the one that we observe. In this experiment, we wish to use a more sophisticated contrast matching strategy to isolate the contribution of the peptide in our data. Specifically, we wish to contrast match the bilayer, such that the scattering is dominated by peptide reorientation. In this way we hope to determine whether the behaviour of the peptide is consistent with the suggested mechanism, or whether a different mechanism occurs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24070910
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24070910
Provenance
Creator Dr Stephen Roser; Dr Rob Barker; Mr Farid Sa'adedin; Dr Arwel Hughes
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-08-04T11:54:51Z
Temporal Coverage End 2009-08-07T07:39:28Z