We have been investigating the influcence of the cationic staphylococcal lipid lysyl-phosphatidylglycerol (L-PG) on bacterial resistance to antimicrobial peptides (AMPs). Using our own novel stable analogue of L-PG (3adLPG), we have examined the effects of increasing 3adLPG in symmetric vesicles upon peptide partitioning in order to model the AMP resistance observed in stapylococci which express high quantities of L-PG when grown at low pH (in environments such as the human nostrils). We propose to examine the effects on both membrane characteristics and peptide interaction of a more appropriate staphylococcal-memitic vescile model. Our model will be composed of asymmetric lipid biayers, the lipid distributions of which will be based upon our own lipidomic data from MRSA. Our vesicles will also have a pH gradient across the membrane in order, analogous with that found in nature.