Phospholipid exchange between tethered bilayers and vesicles

DOI

Our objective is to create asymmetric solid surface tethered bilayers (tBLMs) with the the outer leaflet predominately populated by charged phospholipids. tBLMs are produced by solvent exchange. In such constructs, the distribution of phospholipid material is even. Our preliminary data show possibility to modify composition of the tBLMs via simple vesicle exchange protocols. However, it is unclear, to what extent the asymmetry of membrane may be generated in such a process. Using deuterated phospholipid vesicles we will investigate the equilibrium and dynamics of interaction between the vesicles and non-deuterated tBLMs, in relevant solvents. From the NR changes and calculated neutron scattering length density distributions we will evaluate if and under what condition an asymmetry may be produced.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24088574
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088574
Provenance
Creator Dr Gintaras Valincius; Mr Mindaugas Mickevicius
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-03-07T09:33:56Z
Temporal Coverage End 2012-03-10T18:53:43Z