Introducing the O-antigen into Bacterial Outer Membrane Models

DOI

The Gram negative bacterial outer membrane protects the bacterium from external toxins such as antibiotics. This is partially responsible for Gram negative bacteria becoming the greatest emerging source of antibiotic resistance. We are developing models of these bacterial outer membranes and have successfully recreated the characteristic lipid asymmetry (with lipopolysaccharide (LPS) on the outer leaflet and phospholipids on the inner) and also the water filled periplasm. In order to fully recreate the membranes we will need to incorporate more complex LPS versions to mimic pathogenic bacteria. We will incorporate LPS containing one or two O antigens into the final model at different densities to determine who much it increases the bilayer thickness. In future experimental programmes we aim to increase the LPS length to investigate how very long LPS behave.

Identifier
DOI https://doi.org/10.5286/ISIS.E.84802576
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/84802576
Provenance
Creator Dr Luke Clifton; Professor Jeremy Lakey; Dr Nico Paracini; Dr Maxmilian Skoda
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2017-03-05T09:00:00Z
Temporal Coverage End 2017-03-07T09:00:00Z