Ligand self aggregation is a common problem when studying interactions with bio(macro)molecules. In particular for techniques requiring relatively high ligand concentrations, such as NMR and isothermal titration calorimetry (ITC), data analysis has to take ligand self aggregation into account. We have developed analysis software for ITC data which includes several self aggregation models. We are currently implementing cooperative self aggregation models. In order to validate these models, we require independent measurements of aggregate size as well as information on the shape of the aggregates. The proposed experiments will provide both aggregate size and shape under the required conditions so that our cooperative self aggregation models can be validated.