Effect of End Group on RAFT-Polymers for Lipid Nanodisc Stabilisation

DOI

Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Previously we studied discs formed using a poly(styrene-alt-maleic acid) (PSMA) copolymer, and variants of this material synthesised using RAFT controlled radical polymerisations. The RAFT method results in a C12 chain on the end of the styrene tail of the polymer, from the RAFT agent that controls the polymerisation reaction. Removal of the RAFT endgroup leaves a hydrophilic cyanoisopropyl group, which can be then further functionalised. We have prepared a series of polymers where the endgroup has converted to a cyanoisopropyl group, which, based on DLS measurements, appears to destabilise polymer aggregation in solution and favour stability of larger lipid aggregates. Here we wish to ensure these polymers form discs and probe effects on disc structure.

Identifier
DOI https://doi.org/10.5286/ISIS.E.101134497
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/101134497
Provenance
Creator Dr Luke Clifton; Dr James Doutch; Dr Robert Dalgliesh; Mr George Neville; Professor Karen Edler; Ms Kerrie Morrison
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2019-03-11T00:00:00Z
Temporal Coverage End 2019-03-13T00:00:00Z