Effect of squalene on membrane structure and Amphotericin B resistance in Candida glabrata.

DOI

The yeast Candida glabrata is becoming an increasing cause of infections. We have developed RNA interference tools that allow us to study the virulence and resistance mechanisms in C. glabrata by upregulating/downregulating specific genes, and we determine the corresponding changes in its cell membrane lipid and sterol composition. We recently observed an accumulation of squalene in a C. glabrata strain with resistance to the antifungal drug Amphotericin B (AmB), which acts by binding ergosterol. Our previous neutron reflectivity measurements have shown that reconstituted C. glabrata membranes have different binding affinity for AmB depending on the sterol composition. As squalene may contribute to altered susceptibility to AmB, we propose in this experiment to investigate the effect of squalene on the structure of C. glabrata and POPC lipid bilayers, and their response to AmB.

Identifier
DOI https://doi.org/10.5286/ISIS.E.98002906
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/98002906
Provenance
Creator Mr Juan Manuel Orozco Rodriguez; Dr Maxmilian Skoda; Dr Hanna Wacklin-Knecht; Mr Robin Delhom; Dr Wolfgang Knecht
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2018-09-17T08:00:00Z
Temporal Coverage End 2018-09-26T04:28:14Z