Small-angle neutron scattering study of human growth hormone-surfactant complexes

DOI

Liquid pharmaceutical formulations are commonly comprised of proteins and surfactants, which interact in solution in order to provide an increase in system stability. Although these systems have been significantly investigated in the last few years, there is a lack of consensus concerning the interaction mechanism between proteins and surfactants. We believe that contrast variation small-angle neutron scattering can provide specific information about these systems and contribute to resolving the puzzle. In this experiment we aim to elucidate the interactions between human growth hormone and a prototypical anionic amphiphiles at room temperature. The use of isotope labelling and contrast matching will provide detailed information about individual parts of the system, which will then be co-refined in order to provide structural information of the protein-surfactant complexes as a whole.

Identifier
DOI https://doi.org/10.5286/ISIS.E.99292400
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/99292400
Provenance
Creator Dr Adrian Sanchez-Fernandez; Dr Judith Houston; Dr Sarah Rogers
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-10-24T08:00:00Z
Temporal Coverage End 2018-10-26T08:00:00Z