Influence of polymer molecular weight on polymer stabilized lipid nanodiscs

DOI

Nanostructured lipid aggregates offer enormous potential as tools for membrane structural biology and biophysics. Poly(Styrene-Maleic Acid) stabilized lipid nanodiscs have a number of attractive characteristics including stability, ease of preparation and cost, but questions remain over the mechanism by which the polymer interacts with the lipids to stabilize the nanodiscs. So far our experiments have used a polydisperse commercial polymer. We have now synthesised polymers with well-defined molecular weights using RAFT polymerisation, and also analogues with the styrene component deuterated providing a new set of contrasts in this polymer-lipid system. Here we propose to investigate the effect of polymer molecular weight on the nanodisc size and shape for several lipid compositions, and also, using contrast, to highlight the location and role of the polymer in stabilizing the nanodiscs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24088956
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24088956
Provenance
Creator Professor Karen Edler; Dr Ilaria Idini; Dr Cameron Neylon; Dr Emelie Nilsson; Professor Viveka Alfredsson
Publisher ISIS Neutron and Muon Source
Publication Year 2015
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2012-07-17T07:31:30Z
Temporal Coverage End 2012-07-27T12:36:34Z