Membrane Composition-Dependent Interaction between Beta-Arrestins and Lipid Membranes

DOI

Beta-arrestins are proteins that regulate GPCR signaling, e.g. by recruiting endocytic components to remove the receptor from the plasma membrane. Our fluorescence microscopy and QCM-D studies suggest beta-arrestins are recruited specifically by phosphoinositide lipids, bind membranes in a concentration-dependent manner, and can induce membrane curvature. In order to determine how membrane composition and protein concentration controls interaction between beta-arrestins and membranes, we need to use neutron reflection to characterize protein binding to supported bilayers. This will be achieved by studying membranes composed to isolate different types of interaction, titrating them with a relevant range of protein concentration, and using appropriate contrast matching. These results will let us identify the mechanism of interaction and critical steps in the function of beta-arrestins.

Identifier
DOI https://doi.org/10.5286/ISIS.E.42594214
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/42594214
Provenance
Creator Dr Tania Lind; Professor Kell Mortensen; Mr Achebe Nzulumike; Dr Dimitrios Stamou; Professor Marité Cárdenas Gómez
Publisher ISIS Neutron and Muon Source
Publication Year 2016
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2013-10-14T23:00:00Z
Temporal Coverage End 2013-10-20T16:10:36Z