Small angle neutron scattering study on the interaction between antimicrobial peptides and bacterial endotoxin aggregates.

DOI

Bacterial endotoxins, or lipopolysaccharide (LPS), released from the gram negative bacterial membrane, form different aggregates in solution which morphology depends on the type of LPS expressed by the microbes and have diverse toxicity in humans. Humans produce several antimicrobial peptides which have shown interaction with LPS; this interaction induces structural changes on LPS aggregates, as we reported in our previous SANS experiments.A change in the morphology of the aggregates is connected with a changes in LPS toxicity. We aim to test two human peptides against a wide range of LPS chemotypes in order to assess the efficacy of the interaction depending on the different structures of LPS. Ultimately SANS data will be corroborated by Cryo transmission electron microscopy and biological toxicity test to establish the possible anti-endotoxic role of these human antimicrobial peptides.

Identifier
DOI https://doi.org/10.5286/ISIS.E.42589216
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/42589216
Provenance
Creator Dr Richard Harvey; Mr Yussif Saaka; Ms Helene Marbach; Dr Gianluca Bello
Publisher ISIS Neutron and Muon Source
Publication Year 2016
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2013-09-28T23:00:00Z
Temporal Coverage End 2013-10-01T08:41:02Z