<b>Spatiotemporal mapping of the contractile and adhesive forces sculpting early </b><b><i>C. elegans</i></b><b> embryos</b>

DOI

Embryo shape is determined by individual cell mechanics, intercellular interaction strength, and geometrical constraints. Models based on surface tensions at cell interfaces can predict 3D static cellular arrangements within aggregates. However, predicting the dynamics of such arrangements is challenging due to difficulties in measuring temporal changes in tensions. Here, we characterise the spatiotemporal changes in cellular tensions shaping the early nematode embryo using AFM, live microscopy, and tension inference. Using excoriated embryos, we validate a hybrid inference pipeline that calibrates relative inferred tensions temporally using cortical myosin enrichment and absolute tensions using AFM measurements. Applied to embryos within their native shell, we infer a spatiotemporal map of absolute tensions, revealing that ABa, ABp, and EMS compaction is driven by increased tension at free surfaces, while P2’s initial exclusion is due to high tension at intercellular contacts. We uncover a direct and non-affine contribution of cadherins to cell-cell contact tension, comparable to cadherins’ indirect contribution via actomyosin regulation.This repository contains all of the microscopy raw data and the code to produce most of the figures in the associated publication: https://doi.org/10.1101/2023.03.07.531437 Please consult the "README" document for a full description of how to do this.

Identifier
DOI https://doi.org/10.5522/04/21977771.v1
Related Identifier HasPart https://ndownloader.figshare.com/files/52503626
Metadata Access https://api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:figshare.com:article/21977771
Provenance
Creator Charras, Guillaume; Yamamoto, Kazunori; Goehring, Nathan ORCID logo; Borrego Pinto, Joana; Turlier, Hervé; ichbiah, sacha; delbary, fabrice; Perez, Matthieu
Publisher University College London UCL
Contributor Figshare
Publication Year 2025
Rights https://creativecommons.org/publicdomain/zero/1.0/
OpenAccess true
Contact researchdatarepository(at)ucl.ac.uk
Representation
Language English
Resource Type Dataset
Discipline Basic Biological and Medical Research; Biology; Biophysics; Developmental Biology; Life Sciences; Medicine; Medicine and Health; Physiology