Elucidating the unique role of non-standard collagen helices in forming key protein-protein complexes

DOI

The collagens are the most abundant proteins in mammalian systems, comprising up to 25% of human proteins. Their three-stranded triple helix structures are a major determinant of their function. Our work on basic triple helix structures by SAXS and atomistic modelling revealed previously unknown flexibility and bends in its linear structure. Our efforts to repeat this work for more specialized triple helices in the complement proteins were held up by severe X-ray radiation damage. Test neutron scattering gave excellent results. We will thus compare the structures of basic and specialized triple helical peptides using a combination of neutron and X-ray scattering, ultracentrifugation and advanced atomistic modelling in CCP-SAS. An understanding of these collagen structures will provide essential new insights for understanding the molecular properties of triple helices and their function.

Identifier
DOI https://doi.org/10.5286/ISIS.E.95670390
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/95670390
Provenance
Creator Miss Hina Iqbal; Dr James Doutch; Professor Stephen Perkins
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-06-11T08:00:00Z
Temporal Coverage End 2018-06-12T08:00:37Z