Kinetic Study of the Mechanistic Complexation Process Between DNA and Cationic Polymers

DOI

We propose to use the new SANS2d to study the dynamic structural evolution in the couse of the formation of DNA-cationic polymer complexes. The dynamic process and the mixing will be supported by using an online stopped-flow system. As this is the first time this type of experiment is tried, a substantial part of effort will be devoted to experimental setup and optimisation of the working conditions. The work will be essnetial for us to identify the contributing roles of structure and charge of the complexes on cell transfection efficiency

Identifier
DOI https://doi.org/10.5286/ISIS.E.24087873
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24087873
Provenance
Creator Professor Jian Lu; Dr Sarah Rogers; Dr Xiubo (Jon) Zhao; Ms Amy Freund; Mr Faheem Padia
Publisher ISIS Neutron and Muon Source
Publication Year 2014
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2011-10-10T18:00:09Z
Temporal Coverage End 2011-10-11T09:22:17Z