INTER as a new Tool to Follow the Dynamic Adsorption of Antibody and Non-ionic Surfactant

DOI

Following our very recent work on the dynamic co-adsorption of our control antibody COE-3 and nonionic surfactant PS80-7EO from the mixed solution, We request 3 days of Inter beam time to continue this exciting study by completing parallel runs using deuterated surfactant under solvent contrasts to match out surfactant and antibody separately. In addition, we will carry out similar Inter measurements under sequential adsorption starting from COE-3 followed by surfactant binding. Given our previous work has already revealed the same adsorbed amount and layer structure from the two modes of adsorption under equilibration, our goal here is to unravel the detailed dynamic processes to help understand how mAb deformation or unfolding during interfacial adsorption could induce surfactant binding, resulting in well-organised surfactant bilayer on top of the mAb monolayer...

Identifier
DOI https://doi.org/10.5286/ISIS.E.90586810
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/90586810
Provenance
Creator Mr Ruiheng Li; Mr Sean Ruane; Mr Haoning Gong; Dr Zongyi Li; Dr Lisa Pan; Dr Chris van der Walle; Dr Nina-Juliane Steinke; Mr Robert Holman; Professor Jian Lu
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Natural Sciences; Physics
Temporal Coverage Begin 2018-02-21T08:00:00Z
Temporal Coverage End 2018-02-25T04:20:47Z