Examining the Structural Changes of a Central Nervous System Neurotransmitter Signaling Channel Homolog upon Ligand Binding.

DOI

GluR0 is a glutamate sensitive potassium ion channel from the cyanobacterium Synechocystis which has been identified via primary structural sequencing to be similar to eukaryotic channels involved in neurotransmitter binding (particularly in the mammalian central nervous system). No high or low resolution structure is currently available for the full structure of any member of this important class of signalling proteins. GluR0 opens in the presence of glutamate to allow potassium ions to move across the membrane. We propose studying the structure of this channel solublized in surfactant solutions in both its open and closed conformations using SANS. A contrast variation approach will allow the relative contributions of the surfactant and the protein to be deconvoluted to provide novel information on the relationship of structure and function in this important family of proteins.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24080016
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24080016
Provenance
Creator Dr Cameron Neylon; Dr Luke Clifton; Dr John Collier; Mr Mike Sanders; Mr Gareth Williams; Dr Web Tracks
Publisher ISIS Neutron and Muon Source
Publication Year 2013
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2010-03-09T11:34:05Z
Temporal Coverage End 2010-03-15T09:08:32Z