Purothionin binding to model fungal lipid membranes

DOI

Purothionins and defensins are basic, amphiphilic, cysteine-rich and low molecular weight plant defence proteins. These proteins are active against a broad range of phytopathogenic fungi, andcan be potentially exploited both in terms of environmentally friendly plant fungicides and as future human therapeutic agents. The aim of our research is to investigate the mode of binding of these proteins to model fungal lipid bilayers. We propose to carry out neutron reflectivity experiments at the solid/liquid interface to investigate the influence of membrane unsaturation and the presence of sterols on protein binding, as well as kinetics of protein adsorption. The findings from the NR studies will determine quantitatively the protein-lipid interfacial layer structure and will enable the differences in the proteins' mode of action at membrane surfaces to be investigated.

Identifier
DOI https://doi.org/10.5286/ISIS.E.61002138
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/61002138
Provenance
Creator Ms Olga Florek; Professor Rebecca Green; Dr Luke Clifton; Ms Marleen Wilde; Professor Richard Frazier
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2015-04-15T08:00:00Z
Temporal Coverage End 2015-07-06T08:00:00Z