Relation between surface tension and adsorbed amount of proteins at the air/water interface

DOI

The surface tension of proteins often exhibits a reproducible lagtime, which is sensitiv to a range of conditions such as concentration, pH, ionic strength. We have also found that the time behaviour of mixtures of proteins is quite different from that of the individual proteins. None of this is understood, although there are some theoreticl models available to be tested. We have an accepted experiment to probe the neutron reflectivity and surface tension simultaneously using the individual and mixed components of lysozyme and human serum albumin. Since making that proposal we have had the opportunity of testing out a perdeuterated protein, MPB, maltose binding protein, which has physical characteristics similar to those of HSA. The use of this protein on its own and in mixtures with lysozyme, would make the surface experiment very much more powerful and this is what we now propose.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24078718
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24078718
Provenance
Creator Professor Jeff Penfold; Dr Bob Thomas
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-11-27T09:05:16Z
Temporal Coverage End 2009-11-30T08:39:33Z