Zwitterionic Polymers for Lipid Nanodisc Stabilisation

DOI

Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Previously we studied discs formed using a poly(styrene-alt-maleic acid) (PSMA) copolymer, and also with a commercial poly(styrene-alt-maleimide). However these polymers can be used only over limited pH ranges, as they precipitate when their charge is neutralised. To overcome this we have devised a simple modification of the hydrolysis procedure which produces a zwitterionic version of the copolymer from the same initial poly(styrene-alt-maleic anhydride). The modification can be done to either the widely used commercial polymers, or our controlled Mw copolymers from RAFT polymerisations. Here we wish to measure particles formed using zwitterionic copolymers with DMPC to confirm that they are nanodiscs and to compare to those formed using the acid copolymers

Identifier
DOI https://doi.org/10.5286/ISIS.E.99689448
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/99689448
Provenance
Creator Mr George Neville; Dr James Doutch; Mr Jake Hooton; Ms Kerrie Morrison; Dr Andrew McCluskey; Dr Luke Clifton; Ms Ria Atri; Professor Karen Edler; Dr Marcelo da Silva
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2018-11-14T09:00:00Z
Temporal Coverage End 2018-11-17T09:01:15Z