Copy of: Dynamics of amyloid protein fibril elongation using isotope-labelled SANS

DOI

This proposal combines small-angle neutron scattering with novel deuteration strategies to investigate amyloid protein fibrils. Variations in dynamics of elongation and breakage underpin phenomena ranging from non-genetic heredity in yeast, to diseases such as Alzheimers, BSE and CJD. However, solution methods to monitor growth dynamics only give information on the overall % of material transformed from non-fibrillar to fibrillar forms. They cannot determine elongation rate directly, instead convoluting it with length distribution; and so cannot distinguish a few long fibrils growing quickly from many short fibrils growing slowly. We have developed a SANS approach to measure elongation rate, with isotope labelled ends growing on contrast-matched "seed" fibrils, demonstrated by proof -of-concept data on SANS2D in 2016. This proposal will enable us to finish the study, to allow publication

Identifier
DOI https://doi.org/10.5286/ISIS.E.94114501
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/94114501
Provenance
Creator Mr Ben Eves; Dr Adam Squires; Dr James Doutch; Dr Michael Haertlein; Dr Trevor Forsyth; Dr Ann Terry; Mr Han Yin
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-06-21T08:00:00Z
Temporal Coverage End 2018-06-25T08:06:42Z