We determined nucleosome positions genome-wide in diploid Saccharomyces species undergoing early stages of synchronous meiosis. This study sought to assess if meiotic DNA double-strand break formation occurred preferentially in promoter nucleosome-depleted regions in other Saccharomyces species, as it does in S. cerevisiae SK1 (Pan et al. 2011 Cell 144:719-731). Overall design: Twelve samples were sequenced: three samples of S. cerevisiae YPS128 (two biological replicates, and a technical replicate digested with different amount of MNase), two biological replicates of S. cerevisiae UWOPS03-461.4, three samples of S. paradoxus (two biological replicates, and a technical replicate digested with different amount of Mnase), two biological replicates of S. mikatae, two biological replicates of S. kudriavzevii.