3,606 datasets found

The wrapping of DNA around histone octamers challenges processes that use DNA as their template. In-vitro, DNA replication through chromatin depends on histone modifiers,...

RNA seq was used to compare the transcriptomes of a dcp2-7 strain to dcp2-7 kap123 double mutant and dcp2-7 kap123 tlgag triple mutant

In this work, we determine total mRNA decay rates in rpb1-1 and rpb1-1/caf1? cells, calculate half-lives in rpb1-1/caf1? cells relative to rpb1-1 cells and correlate them with...

We analyze genome-wide gene expression response of Saccharomyces cerevisiae to sequential reduction of RAP1 levels. Overall design: We compared yeast with wildtype RAP1 level...

We have isolated and microsatellite typed hundreds of S. cerevisiae strains from spontaneous fermentations of grapes from the Okanagan Valley wine region in British Columbia...

Lignocellulosic biomass is still being looked into as a feasible source of bioethanol production. The yeast Saccharomyces cerevisiae can adapt to in situ detoxify...

The Origin Recognition Complex (ORC) seeds replication-fork formation by binding to DNA replication origins, which in budding yeast contain a 17bp DNA motif. High resolution...

Hydrogen sulfide (H2S), a traditionally known cytotoxic gas, has been recently included in the gasotransmitters family. Previous studies demonstrated that lifelong treatment...

Accurate gene expression requires the coordination of RNA processing with assembly of messenger RNA-protein (mRNP) complex. RNA helicases are a class of enzymes that unwind RNA...

The aim of this study is to evaluate how cellular energy levels shape codon-specific decoding and affect codon-mediated mRNA degradation. To this end we made use of HT5P-seq...

Ribosome stalling occurring on aberrant mRNA activates quality control pathways to maintain proteostasis. Recently, ribosome stalling has also been linked to the activation of...

Translation factors eIF4E and eIF4G form eIF4F, which binds to mRNAs to promote ribosome recruitment and translation initiation. We were interested in analysing the effects of...

In Saccharomyces cerevisiae the forkhead (Fkh) transcription factor Fkh1 forkhead homolog) enhances the activity of many DNA replication origins that act in early S-phase (early...

To study the relevance of the phosphorylation of Spt4 in residues T42 and S43, we assessed the genome-wide transcriptional response of the non-phosphorylatable Spt4-T42A/S43A...

We used RNA-seq to monitor mRNA levels of all genes in response to hypoxia of wild-type yeast, S. cerevisiae (strain yMH914 with wildtype HAP1). To gain insights into how gene...

RNAseq profiling of engineered S. cerevisiae during conversion of cellulosic sugars into biofuels Sample from Aerobic bioreactor containing cellulosic media

Heterologous expression of nrps33, a nonribosomal peptide synthetase gene, from Paecilomyces cinnamomeus BCC 9616 in Saccharomyces cerevisiae YPH 499 unexpectedly resulted in...

Proper formation of messenger RNA-protein complexes (mRNPs) is critical for accurate gene expression and requires temporal regulation of mRNA structures. RNA helicases are major...

Biological innovations have been considered to be drive by functional and transcriptional divergence between copies after gene duplication events. Here we present the exhaustive...

Foldback priming at naturally occurring inverted repeats is proposed to initiate formation of large, inverted duplications, the major class of chromosome rearrangement recovered...