These data supplement the article “Aquatic biofilms as passive environmental DNA samplers: application to benthic macroinvertebrate communities in rivers” Sinziana F. Rivera, Valentin Vasselon, Nathalie Mary, Olivier Monnier, Fréderic Rimet & Agnès Bouchez submitted to “Molecular Ecology Resources” journal.------------------ The directory is composed of: “38_samples_fastq:files”: contains raw demultiplexed fastq files (R1. fastq and R2. fastq) for each of the 38 samples used in this study to produce OTUs and taxonomic inventories.----------------------------- “Samples id.xlsx”: contains the samples ID of the fastq files---------------------------- “Inventories.xlsx”: contains single and multi-habitat morphological inventories as well as molecular inventories resulting from the study