Response of Arctic benthic bacterial deep-sea communities to different detritus composition during an ex-situ high pressure experiment

DOI

In a multidisciplinary ex situ experiment, benthic bacterial deep-sea communities from 2,500 m water depth at the Long-Term Ecological Research Observatory HAUSGARTEN (stationPS93/050-5 and 6), were retrieved using a TV-guided multiple corer. Surface sediments (0 - 2 cm) of 16 cores were mixed with sterile filtered deep-sea water to a final sediment dilution of 3.5 fold. The slurries were split and supplemented with five different types of habitat-related detritus: chitin, as the most abundant biopolymer in the oceans, and four different naturally occurring Arctic algae species, i.e. Thalassiosira weissflogii, Emiliania huxleyi, Bacillaria sp. and Melosira arctica. Incubations were performed in five replicates, at in situ temperature and at atmospheric pressure, as well as at in situ pressure of 250 atm. At the start of the incubation and after 23 days, changes in key community functions, i.e. extracellular enzymatic activity, oxygen respiration and secondary production of biomass (bacterial cell numbers and biomass), were assessed along with changes in the bacterial community composition based on 16S rRNA gene and 16S rRNA Illumina sequencing. In summary, differences in community structure and in the uptake and remineralization of carbon in the different treatments suggest an effect of organic matter quality on bacterial diversity as well as on carbon turnover at the seafloor.

Supplement to: Hoffmann, Katy; Hassenrück, Christiane; Salman-Carvalho, Verena; Holtappels, Moritz; Bienhold, Christina (2017): Response of bacterial communities to different detritus compositions in Arctic deep-sea sediments. Frontiers in Microbiology, 8, 266

Identifier
DOI https://doi.org/10.1594/PANGAEA.867475
Related Identifier IsSupplementTo https://doi.org/10.3389/fmicb.2017.00266
Related Identifier References https://doi.org/10.2312/BzPM_0693_2015
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.867475
Provenance
Creator Hoffmann, Katy ORCID logo; Hassenrück, Christiane ORCID logo; Salman-Carvalho, Verena; Holtappels, Moritz ORCID logo; Bienhold, Christina ORCID logo
Publisher PANGAEA
Publication Year 2016
Funding Reference Seventh Framework Programme https://doi.org/10.13039/100011102 Crossref Funder ID 294757 https://cordis.europa.eu/project/id/294757 Assessment of bacterial life and matter cycling in deep-sea surface sediments
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 1540 data points
Discipline Earth System Research
Spatial Coverage (4.181 LON, 79.065 LAT); North Greenland Sea