Quantifying antibiotic resistance using bacterial model membranes

DOI

The Gram negative bacterial outer membrane protects the bacterium from toxins such as antibiotics and is partially responsible for the rapid rise in antibiotic resistance in these bacteria. Its outer surface is formed by a lipid, lipopolysaccharide (LPS) which is unique to Gram negative bacteria. We are developing models of these bacterial outer membranes and have successfully recreated their interaction with the antibiotic Polymyxin B which acts directly upon LPS . We can now observe the effects of natural LPS modifications which occur in resistant bacteria and here we wish to study the effect of reduction in LPS negative charge. We will observe how it reduces antibiotic binding and also its cost to the viability of the bacterium, in terms of membrane stability. This will enable the design of better antibiotics effective against antibiotic resistant bacteria.

Identifier
DOI https://doi.org/10.5286/ISIS.E.99692847
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/99692847
Provenance
Creator Professor Jeremy Lakey; Dr Nico Paracini; Dr Maxmilian Skoda; Dr Luke Clifton
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2018-12-02T09:00:00Z
Temporal Coverage End 2018-12-05T09:00:49Z