Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle Data and scripts

DOI

The ZIP files contain analysis files (Python) for the figures, as well as files for the two used models, i.e. the cell mass model and the FBA model. The analysis is described in detail in the related publication. (2023) Specifically, the different ZIP files contain:

Figure1.zip: - Data and script for analysis karyokinesis time; - Data and script for analysis protein synthesis rate with stop-and-respond method (replicate 1); - Data and script for analysis protein synthesis rate with stop-and-respond method (replicate 2); - Data and script for analysis protein synthesis rate with sfGFP. 

Figure2.zip: - Data and script for cell surface and cell volume dynamics; - Data and script for analysis lipid synthesis rate (replicate 1); - Data and script for analysis lipid synthesis rate (replicate 1); - Data and script for analysis polysaccharide synthesis rate (replicate 1); - Data and script for analysis polysaccharide synthesis rate (replicate 2); - Data and script for control analysis polysaccharide synthesis rate.

Figure3.zip: - Cell mass model.

Figure4.zip: - Cell cycle FBA model; - Data and script for experiment with glucose analog uptake (replicate 1); - Data and script for experiment with glucose analog uptake (replicate 2); - Data and script for glycolytic flux sensor experiment.

Figure5.zip: - Data and script for analysis of NADPH oscillations; - Data and script for microaerobic conditions and storage mutants.

Identifier
DOI https://doi.org/10.34894/XPYC7Y
Related Identifier https://doi.org/10.1038/s42255-023-00741-x
Metadata Access https://dataverse.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34894/XPYC7Y
Provenance
Creator Takhaveev, Vakil ORCID logo; Özsezen, Serdar; Smith, Edward N.; Zylstra, André; Chaillet, Marten; Chen, Haoqi ORCID logo; Papagiannakis, Alexandros (ORCID: 0000-0002-6363-804X); Milias-Argeitis, Andreas ORCID logo; Heinemann, Matthias ORCID logo
Publisher DataverseNL
Contributor Groningen Digital Competence Center
Publication Year 2023
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Groningen Digital Competence Center (University of Groningen)
Representation
Resource Type Dataset
Format application/zip
Size 23338776; 20718890; 10744908; 46602943; 1580899
Version 1.1
Discipline Chemistry; Life Sciences; Medicine; Natural Sciences