SOLVENT-MATCHING SANS ON COMPLEXES FORMED BETWEEN DNA AND SHORT AMPHIPHILIC PEPTIDES

DOI

We will perform SANS investigations on complexes formed between peptide amphiphiles (PAs) and the plasmid DNA pEGFP-N1. Previous SAXS data have shown that our PAs form nanoscopic fibrils or layered structures, thus potentially able to host DNA. We propose solvent matching experiments where D2O/H2O ratio will be varied to separately match the contrast of PAs and DNA. Our data will be combined with cell assays following transfection with the peptide/plasmid complexes to determine a relationship between structure and delivery.

Identifier
DOI https://doi.org/10.5286/ISIS.E.58451608
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/58451608
Provenance
Creator Mr Steven Kirkham; Professor Ian Hamley; Dr Emerson SILVA; Dr Steve King
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2015-03-23T09:00:00Z
Temporal Coverage End 2015-03-26T09:00:00Z