Double-digest RAD Sequencing using Ion Proton semiconductor platform (ddRADseq-ion) with non-model organisms

We have developed a new method for generating single nucleotide polymorphisms (SNPs) using next-generation sequencing. RAD Sequencing has become an established method for SNP genotyping on Illumina sequencing platforms. With a modification to the double-digest RAD Sequencing methodology on Illumina, we have developed a protocol and new adapters for using Ion Torrent semiconductor sequencing. To validate and demonstrate the method, we sequenced 13 genomic libraries of three non-model vertebrate species (two fishes and a lizard).

Identifier
Source https://data.blue-cloud.org/search-details?step=~01277815B087C7FD3B6EF770F59CD6E3E346472FCD9
Metadata Access https://data.blue-cloud.org/api/collections/77815B087C7FD3B6EF770F59CD6E3E346472FCD9
Provenance
Instrument Ion Torrent Proton; ION_TORRENT
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2015-03-24T00:00:00Z