Comparative Interaction Studies of Antimicrobial Agents on Floating Asymmetric Bacterial Membrane Models

DOI

The outer membrane (OM) of Gram-Negative bacteria (GNB) is an asymmetric structure which contains complex lipids known as lipopolysaccharides (LPSs) on the outer surface. LPSs comprise the membrane anchoring Lipid-A, the anionic core oligosaccharides and the very large, solution facing O-antigen which acts as a hydrophilic barrier to antibiotics. We have recently achieved an accurate model of the outer membrane using short chain LPS and now wish to start examining its interaction with antimicrobial peptides and proteins. These will include the antibiotic polymyxin, human innate immune peptide cathelicidin, LPS specific bacteriocin, colicin N and a beta defensin.

Identifier
DOI https://doi.org/10.5286/ISIS.E.81736155
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/81736155
Provenance
Creator Dr Anton Le Brun; Dr Luke Clifton; Dr Christy Kinane; Professor Jeremy Lakey; Dr Stephen Holt; Dr Nico Paracini
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-07-14T07:00:00Z
Temporal Coverage End 2016-07-18T07:00:00Z