Neutron scattering and selective deuteration for elucidating how lipids regulate metabolon formation

DOI

Plants biosynthesise valuable pharmaceutical compounds by a series of enzyme complexes (or metabolons) that work in a concerted action. Metabolons enhance substrate channeling and prevent leakage of toxic or labile intermediates and metabolic cross-talk. Extracting pure products from plats constitutes a great industrial and societal challenge, and bioengineering of moss and bacteria was come up as a very interesting way to produce these compounds. Here, we will dissect the mechanisms by which plants guide the assembly of multi-enzyme complexes. We will use synthetic biology and neutron scattering to map the lipid-protein interactions that control the formation of the dhurrin metabolon, as a model system. This will enable rational enzyme design for metabolons in heterologous hosts to boost the production of bioactive compounds. This experiment will provide the first basis for such studies

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1920128-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/105599867
Provenance
Creator Dr James Doutch; Dr Tomas Laursen; Dr Rita Del Giudice; Professor Marité Cárdenas Gómez; Dr Christopher Garvey; Mr Artur Glavic; Dr Nico Paracini; Professor Max Wolff
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-10-13T07:30:00Z
Temporal Coverage End 2019-10-14T07:55:29Z