Structural determination of SMA-lipid particles in solution

DOI

The development of styrene-maleic acid lipid particles (SMALP) is opening the door to better understanding the nature of membrane proteins, these particles have been well characterised with non-atomistic techniques. However, the protein-free system is still not completely understood atomistically, with information such as the polymer conformation and the lipid arrangement still unknown. The ability of NIMROD to give atomic resolution on this organised system would allow the atomistic structure to be understood. Due to the large size of the system, we plan to use a pre-existing all-atom model as a starting point for the ESPR analysis, to assist in more rapidly determine the experimental solution structure. By comparing SMALPs formed using two different polymers we aim to understand polymer effects on encapsulated lipids which may impact properties of proteins supported in the nanodiscs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.82354929
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/82354929
Provenance
Creator Dr Ann Terry; Ms Cecilia Tognoloni; Dr Andrew McCluskey; Dr Joseph Curtis; Dr Daniel Bowron; Professor Karen Edler
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Chemistry; Natural Sciences
Temporal Coverage Begin 2016-10-04T08:00:00Z
Temporal Coverage End 2016-10-07T08:00:00Z