The solution structure of E.coli peripheral membrane protein FtsA solubilised within a polymer stabilised nanodisc

DOI

We will measure the solution structure of a protein complex from bacteria essential for cell division. The two proteins, FtsA and FtsZ, are known to interact with each other with FtsZ forming a constricting ring, and FtsA being a membrane protein anchoring this ring to the membrane. Furthermore, we have prepared these proteins in a novel way using SMALPs: discoidal structures solubilising membrane proteins within a native lipid environment without the use of detergents which are a poor substitute for the natural lipid bilayer. While interactions are known, the structural details of the assembly are unknown. We propose to use small angle neutron scattering on SANS2D to measure the solution structure of this complex isolated in the most native-like state by using SMALPs, which is essential for the development of novel antibiotics and understanding the mechanism of bacterial cell division.

Identifier
DOI https://doi.org/10.5286/ISIS.E.87761537
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/87761537
Provenance
Creator Professor Karen Edler; Dr Stephen Hall; Dr Najet Mahmoudi; Dr Andrew McCluskey; Dr Thomas Arnold; Dr Luke Clifton; Dr Tim Knowles; Dr Ian Cadby; Dr Mark Jeeves
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2017-09-28T08:00:00Z
Temporal Coverage End 2017-10-02T08:38:53Z