Coastal marshes (Louisiana) Targeted Locus (Loci) Raw sequence reads

This study was done to evaluate changes in microbial communities from coastal marsh subtidal sediments, inland soils, and water in southern Louisiana. These marshes were impacted by the Gulf of Mexico oil spill in 2010, and this project is a continuation and extension of PRJNA232457 (from 2010 - 2013). New samples were acquired from core locations as part of the Coastal Waters Consortium, from June 2012 through October 2014. Bacterial communities were assessed from subtidal sediments collected at 1-2 m from the marsh vegetation edge or from soils collected 5 m inland from the edge (labelled with IN). Water samples were collected from the edge (labeled with EW). Sediment and soil were collected from push cores and sectioned in the field at (A) 0-1 cm, (B) 1-2 cm, (C) 4-5 cm, and (D) 9-10 cm depth intervals. From extracted DNA, the V1-V3 region of the bacterial 16S rRNA gene was amplified and single-direction reads were generated using multiplexed, bar-coded primers for pyrosequencing.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0120AC60FE6FDD01B707A504CD55D1878CCF224986C
Metadata Access https://data.blue-cloud.org/api/collections/0AC60FE6FDD01B707A504CD55D1878CCF224986C
Provenance
Instrument 454 GS FLX Titanium; LS454
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor University of Tennessee
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-90.605W, 29.266S, -89.750E, 29.491N)
Temporal Coverage Begin 2012-06-12T00:00:00Z
Temporal Coverage End 2014-10-12T00:00:00Z