Design and Evaluation of Illumina MiSeq compatible 18S rRNA Primers for Improved Characterization of Mixed Phototrophic Communities.

A systematic analysis of the most commonly amplified 18S rRNA hypervariable regions and the effect that PCR/sequencing bias has on community representation. The V4 and V8-V9 hypervariable regions were amplified for seven mock communities and for freshwater, marine, and wastewater samples to compare the sequenced community structure to theoretical communities and validate these results with environmental samples.

Identifier
Source https://data.blue-cloud.org/search-details?step=~01201882DDD10549EACA2B114B55783FFEA35F9FA1C
Metadata Access https://data.blue-cloud.org/api/collections/01882DDD10549EACA2B114B55783FFEA35F9FA1C
Provenance
Instrument 532; 308
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2026
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-117.700W, 29.653S, -70.980E, 42.400N)
Temporal Coverage Begin 2014-09-13T00:00:00Z
Temporal Coverage End 2014-10-23T00:00:00Z