Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However, modern amplicon data analysis employs methods that may be less sensitive to such artifacts. Here we directly compare results from single versus triplicate reactions for 16S amplicon sequencing and find no significant impact of adopting a less labor-intensive single-reaction protocol.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0125658D59CAEEBFF5A13E03195EEE09442840512CA
Metadata Access https://data.blue-cloud.org/api/collections/5658D59CAEEBFF5A13E03195EEE09442840512CA
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor University of California San Diego Microbiome Initiative;UCSDMI
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-122.420W, 32.500S, -79.770E, 41.840N)
Temporal Coverage Begin 2019-02-15T00:00:00Z
Temporal Coverage End 2019-02-17T00:00:00Z