-
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,... -
Naturally-diverse aerobiology and mice gut microbiota
We subjected 54 x 3-5 week old inbred BALB/C mice (27 female, 27 male) to a 7 week exposure to airborne environmental microbiota treatments in the form of light soil dust, using... -
Microbiome of cotton towels used in daily life
To reveal microbiota of towels used in daily life, a 6-month longitudinal test was conduved. New cotton towels were distributed to 26 households. The towels were collected every... -
Environmnental metabarcoding multiplex trial
Environmental DNA metabarcoding data from different environmental matrices amplified with 16S, 18S and rbcL as singleplexes or combined into a multiplex. -
Unveiling the Ecological Applications of Ancient DNA from Mollusk Shells
The shells of marine mollusks represent promising metagenomic archives of the past, adding to bones, teeth, hairs, and environmental samples most commonly examined in ancient... -
Analysis of dust samples from the Russian part of the ISS
Our study focuses on the hardiest microorganisms inhabiting the ISS, in order to assess their diversity, and capabilities to resist certain stresses. We specifically selected... -
Airborne bacteria confirm the pristine nature of the Southern Ocean boundary ...
Microorganisms are ubiquitous and highly diverse in the atmosphere. Despite airborne bacteria over the Southern Ocean (SO) potentially impact to ecology in isolated Antarctica... -
Interactive effects of herbivory, nutrient enrichment, and temperature on cor...
This is the public repository for any sequencing data generated under NSF award OCE-2023424 granted to Deron E. Burkepile, Rebecca Vega Thurber, and Thomas C. Adam. This project... -
Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene...
A comparison of non-targeted (shotgun metagenomics) sequencing methods and targeted amplicon methods for the characterization of ancient human oral microbiomes. -
MOSAiC aerosol and related source sample amplicon sequencing data
This is the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) expedition aerosol and source sample DNA fastq files for samples collected in the...
