ChIP-seq analysis of FfmA binding in Aspergillus fumigatus

Two strains were analyzed by ChIP-seq to determine the genomic binding sites for the Aspergillus fumigatus transcription factor FfmA. A wild-type strain (AfS35) was subjected to ChIP-seq analysis using an antibody directed against the wild-type version of the FfmA protein. An strain containing an epitope-tagged version (FLAG-ffmA) of the ffmA gene under control of a doxycycline off (dox off) promoter was also analyzed by ChIP-seq. In this latter case, an anti-FLAG antibody was used. The negative controls in both cases consisted of a ChIP-seq reaction with the respective primary antibody omitted. Overall design: ChIP-Seq

Identifier
Source https://data.blue-cloud.org/search-details?step=~01285A1F333F6EF513FC220FCDF652200E053A7F809
Metadata Access https://data.blue-cloud.org/api/collections/85A1F333F6EF513FC220FCDF652200E053A7F809
Provenance
Instrument Illumina HiSeq 2500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Molecular Physiology & Biophysics, University of Iowa
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science