Dynamics of proteins undergoing cold denaturation

DOI

Pressure as a variable in biophysics/biochemistry allow the structure/function and dynamics/function relationships to be analysed in detail in various biomolecular assemblies. Overall volume changes of the biomolecule favour the dissociation of ionic interactions under pressure. Exposure to water of hydrophobic residues occurring, for instance, during protein unfolding disturbs the "loosely packed" structure of pure water and is thus favoured at high pressures. This proposal aims at investigating important aspects of the structure-to-function relationship of a well known and characterised enzyme, lysozyme, using pressure as thermodynamic variable. We are interested in investigating the evolution of the dynamics associated to structural changes, in both native and molten globule configuration, when hydrostatic pressure is applied in the range 1 - 7Kb at room temperature.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24068596
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24068596
Provenance
Creator Professor Sue Kilcoyne
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-06-03T05:39:18Z
Temporal Coverage End 2009-06-10T06:28:26Z