Nitrous oxide production kinetics from ammonia oxidation in the Eastern Tropical North Pacific in March/April 2018

In March/April 2018 during a cruise on R/V Sally Ride, SR1805, 15N-NH4+ incubations in 60mL glass serum bottles were performed to measure ammonium oxidation rates to nitrite and nitrous oxide in different depth at 3 different stations in the oxygen deficient zone (ODZ) of the Eastern Tropical North Pacific off the coast of Mexico. Water samples were collected from 30L Niskin bottles deployed with a conductivity-temperature-depth profiler (CTD, Seabird Electronics). The goal was to get a better understanding on the controls of nitrous oxide (N2O) production. The N2O production rate experiments were performed according to Bourbonnais et al. 2021 ( Furthermore, ammonium (NH4+), nitrite (NO2-) and nitrate (NO3-) as well as N2O concentrations were determined using standard fluorometric (Holmes et al. 1999,, photometric (Strickland and Parsons 1972, hdl:10013/epic.46454.d001), chemiluminescent (Braman and Hendrix 1989, doi:10.1021/ac00199a007) and mass spectrometric techniques (McIlvin and Casciotti 2010,, respectively. The N2O yield per nitrite produced was calculated. The archaeal ammonia monooxygenase gene subunit A (amoA) copy numbers/mL were determined using qPCR as described previously (Peng et al. 2015,

The data table contains the depth profiles of salinity, temperature (°C), oxygen concentration (µmol/L), ammonium (µmol/L), nitrate (µmol/L), nitrite (µmol/L) and nitrous oxide concentration (nmol/L) for three stations in the oxygen deficient zones (ODZ) in the Eastern Tropical North Pacific. It also contains the rate measurements of N2O production rate (nmol N2O/Ld) with standard error, ammonium oxidation rate (nmol N2O/Ld) to nitrite with standard error as well as N2O yield (%) with standard deviation. The % hybrid N2O production refers to the production of 45N2O, which requires incorporation of a 14N substrate other than NH4+. It is expressed in percent with respect to the total N2O produced from 15NH4+. Furthermore, the archaeal amoA copy numbers / mL and the standard deviation are given for the different depths.

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Metadata Access
Creator Frey, Claudia (ORCID: 0000-0002-3544-3800); Sun, Xin (ORCID: 0000-0003-0280-4283); Szemberski, Laura; Casciotti, Karen L (ORCID: 0000-0002-5286-7795); Garcia-Robledo, Emilio (ORCID: 0000-0002-4337-6433); Jayakumar, Amal (ORCID: 0000-0002-3568-1403); Kelly, Colette; Lehmann, Moritz F (ORCID: 0000-0003-0626-5942); Ward, Bess B (ORCID: 0000-0001-7870-2684)
Publisher PANGAEA
Publication Year 2021
Funding Reference National Science Foundation, 1657663; National Science Foundation, 1657868
Rights Creative Commons Attribution 4.0 International;
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 796 data points
Discipline Earth System Research
Spatial Coverage (-113.000W, 10.000S, -102.350E, 17.683N); North Pacific Ocean
Temporal Coverage Begin 2018-03-19T15:28:17Z
Temporal Coverage End 2018-04-08T15:16:35Z