Increased environmental pressures and awareness is promoting a major drive towards an increased use of biosurfactants to produce more biosustainable and biodegradable consumer products, in home and personal care products, cosmetics and foodstuffs. There is also a strong drive towards new structures and functionalities. Recent NR studies on the highly surface active hydrophobin and the food grade polysorbate surfactant (Tween) mixtures show spectacular surface multilayer formation and a different control of wetting properties. We propose to extend these preliminary measurements with more detailed measurements with different isotopic labelling, in order to understand the interactions responsible for the formation of these surface structures.